Abstract

Hematological alterations induced by glyphosate and ameliorative effect of ascorbic acid in wistar rats

Authors: M. Lakshmi Namratha, M. Lakshman, M. Jeevanalatha and B. Anil Kumar

DOI: http://dx.doi.org/10.71081/cvj/2021.005

Abstract
This experimental research was executed to record the adverse toxic effects of glyphosate (GLP) on blood of male albino Wistar rats and its mitigation with administration of Ascorbic Acid (AA). We used a total of 40 adult male rats of similar body mass and age. All the rats were blindly divided and placed in 4 different groups and each experimental group contained12 rats. The rats in group 2 received glyphosate @ 500 mg/kg body weight, rats kept in group 3 received only ascorbic acid @ 250 mg/kg body weight and the rats of group 4 were exposed to glyphosate (500 mg/kg b.wt) and ascorbic acid (250mg/kg b.wt) in combination orally for period of 21 days. All the treatments were given to rats daily by oral route. A total of 6 rats were arbitrarily picked from each group on day 7 and 21 for blood collection. The blood samples were then used for determination of total red blood cells, total white blood cells, hemoglobin (Hb) quantity, hematocrit values and different other blood parameters. The results on blood profile of treated rats exhibited a significant (P<0.05) reduction in the mean values of erythrocyte count, white blood cell count, Hb, haematocrit, MCHC, MCV and MCH in comparison to unexposed rats. Results exhibited that the administration of ascorbic acid (Vitamin C) lowered the adverse toxic ailments of glyphosate on blood of treated rats. In conclusion, the results of this experimental trial suggested that vitamin C partially mitigate the toxic effects induced by glyphosate in rats.

Keywords: Hematology, Glyphosate, Ascorbic acid, albino Wistar rats