Abstract

Evaluation of hematological, antioxidant enzymes and oxidative stress parameters in buffaloes infected with babesiosis

Authors: Zeeshan Ahmad Bhutta, Muhammad Fakhar-e-Alam Kulyar, Jahanzaib, Iqra Sarwar, Samina Shabbir, Prerona Boruah and Abubakar Bello

DOI: http://dx.doi.org/10.71081/cvj/2022.004

Abstract
This study aimed to determine how the babesiosis infection affected hematology, oxidative stress, and antioxidant enzymes in buffaloes. A total of 41 babesia positive and 10 healthy buffaloes having different ages were investigated based on parasitemia. Blood and serum samples were collected from positive and healthy buffaloes for the laboratory examination. Animals had different clinical signs including pyrexia (104°F ± 1.0°F), chocolate color urine, edema of face and legs in 2-3 cases, congested and hyperemic eyes, lethargy, emaciation, watery exudate from eyes and pale mucous membrane of eyes were observed. Results did not show a significant association between buffaloes' age in occurrence of parasitemia. Results on hematological values indicated significantly increased total leukocyte counts, basophil counts, eosinophil and neutrophil counts while significantly reduced values of red blood cell counts, hematocrit, hemoglobin, monocyte and lymphocyte counts in infected buffaloes. The results regarding different antioxidant enzymes in babesia positive cases showed a significantly lower quantity of serum total proteins, albumin concentration, catalase, reduced glutathione and superoxide dismutase as compared to non-infected animals. Results on some oxidative stress parameters indicated significantly higher values of nitric oxide scavenging activity and malondialdehyde quantity in positive buffaloes. In conclusion, the findings of this study showed that babesiosis infection remarkably influences the hematology, oxidative stress and antioxidant enzymes in buffaloes.

Keywords: Buffaloes Babesiosis, Hematology, Antioxidant enzymes, Oxidative stress