Abstract

Bactericidal activity of Chlorine Dioxide on Escherichia coli in water ponds at animal farms

Authors: Sana Akhtar, Muhammad Tahir Sarfraz Khan, Huma Asghar, Tayyaba Javaid and Muhammad Shoaib

DOI: http://dx.doi.org/10.71081/cvj/2022.014

Abstract
Escherichia coli (E. coli) belongs to the bacterial species and causes hazardous effects on health of humans, animal and even on plants that need to be eliminated. Due to chlorine dioxide's effectiveness in disinfecting water and acting as a powerful oxidizer, it has taken the place of chlorine in many applications. This study aims to assess the chlorine dioxide (CLO 2) disinfectant's bactericidal activity against E. coli in water ponds at animal farms. To isolate the E. coli, water samples were gathered from several locations and cultivated on the MacConkey agar. Purified colonies were taken to enrich the bacteria and preserve the bacteria in 50% glycerol. Stock solution was used to make dilutions to check the efficacy of chlorine dioxide. Different concentrations of ClO 2 were tested at different exposure time ranges from 3-14 min. The decreased ratio of viable cells of bacteria in the suspension at different amount of chlorine dioxide was determined. Values were calculated to determine how much the E. coli population was decreased at each sanitizer concentration in aqueous solution. Additionally, under the same experimental settings, linear functions of the log of bacteria inactivation (log 90 percent killing time vs. log concentration of disinfectant) were plotted to test the effectiveness of ClO2. The outcomes were manually recorded.

Keywords: Bactericidal activity, Chlorine dioxide, Escherichia coli, Oxidizer, Animal farm