Abstract

Anti-inflammatory, immunomodulatory and antioxidant activities of Allicin, Vitamin C and Doxycycline, or their combination against Pasteurella multocida infection in Rabbits

Authors: Muhammad Imran, Tazmeen Umer, Habib Ur Rehman, Mudassir Saleem, Haidar Farooq, Zeeshan Ahmad Bhutta, Muhammad Fakhar-e-Alam Kulyar, Samina Shabbir, Prerona Boruah and Juon Abbass

DOI: http://dx.doi.org/10.71081/cvj/2022.015

Abstract
Pasteurella multocida (P. multocida) is a Gram-negative rod-shaped coccobacilli with non- lactose fermenting property. This study examined the potential efficacy of allicin, vitamin C, and doxycycline in reducing the intensity of P. multocida infection in rabbits. 20 locally bred, adult rabbits were divided into five equal groups. Different treatments such as allicin, vitamin C and doxycycline were applied in all groups. Rabbits in group 1 and 2 were kept as control and did not receive any treatment. Group 3 was treated with 500mg allicin and similarly, group 4 and group 5 treated with 100mg doxycycline and combined treatment like allicin 500mg, 100mg, doxycycline and 100mg Vitamin C respectively. Challenge and protection were given after one week in all groups. Except group 1, all remaining groups of rabbits were infected (intranasal) with P. multocida 2×104 CFU ?? ml. Antioxidative activity, biochemical assay, hematological and immunological assays were performed. The results showed significant decrease in count of lymphocytes, hemoglobin and RBCs in infected group with increased phagocytic percentage. Serum biochemical enzyme including ALT, ALP and LDH level were significantly increased in infected group. SOD level showed significant increase in combined treated group and significant decrease in infected group. Sera were collected and titrated for antibody titers through indirect hemagglutination assay (IHA). Results were subjected and data was analyzed by 2 factorial CRD. The results depicted significantly higher IHA titers in group 5 followed by group 3 and 4 with short term sustainability. Rabbits in group 3 treated with 500mg allicin presented promising results than the group 4 rabbits treated with doxycycline. Hence, concluded that the use of antibiotics like doxycycline did not provide maximum protection against P. multocida. While the synergistic effect of allicin, vitamin C and doxycycline showed maximum protection providing evidence for promising remedy for HS.

Keywords: Allicin, Antioxidative enzyme, Doxycycline activity, Hematological, Hemorrhagic septicemia, Immunological assay, Serum biochemical, Vitamin C