Abstract

Isolation, molecular characterization and antimicrobial susceptibility testing of Pseudomonas aeruginosa from skin infection of dogs

Authors: Hira Ahsan, Zeeshan Nawaz, Bilal Aslam, Mehraj Gul, Maryam Shahid and Abu Baker Siddique

DOI: http://dx.doi.org/10.71081/cvj/2022.010

Abstract
Pseudomonas aeruginosa represents one of the most frequently found bacteria on canine skin, and it has been identified as a zoonotic pathogen that spread to human and cause life threatening infections. It is a significant opportunistic pathogen that is challenge to control due to its vast ecological dispersion, inherent resistance to various classes of antimicrobial drugs, high potential of developing novel resistance mechanisms and broad selection of pathogenic causes. The main goal of this research was to detect the frequency of Pseudomonas aeruginosa isolated from skin infection of dogs and their susceptibility pattern against antipseudomonal antibiotics. Total of one hundred and twenty skin swabs were collected from infected dogs which were then applied to isolate and identify Pseudomonas aeruginosa by applying standard microbiological methods. Molecular characterization of Pseudomonas aeruginosa was performed via PCR by utilizing specie specific primers against oprL and Oprl genes of Pseudomonas aeruginosa. Antimicrobial susceptibility testing was performed by Disc diffusion assay. Out of 120 skin samples, 24(20%) were identified as Pseudomonas aeruginosa. Molecular detection showed 100% positive results for Pseudomonas aeruginosa. Antimicrobial resistant pattern showed highest resistance of isolates to Cefepime (70%), while 50% susceptibility of isolates was found to the Colistin. Out of 24 isolates of Pseudomonas aeruginosa, 14(58%) were considered as MDR Pseudomonas aeruginosa. In conclusion there is an imperative need for ways to regulate and stop the emergence of novel multidrug-resistant microorganisms.

Keywords: Pseudomonas aeruginosa, Multidrug resistant, Antimicrobial susceptibility, Dog, Zoonosis