Abstract

Assessment of third generation cephalosporin resistant Escherichia coli in raw beef samples from retail market of Faisalabad

Authors: Faisal Ali, Prerona Boruah and M. Imran Arshad

DOI: http://dx.doi.org/10.71081/cvj/2023.005

Abstract
Beef is a crucial source of protein, vitamins and daily require nutrients. To maintain its safety and quality constant monitoring is required. Because of the higher danger of foodborne diseases, meat is a major issue globally related to health because of contamination of the meat through different bacterial contaminants. Escherichia coli is mostly present globally in livestock, particularly cattle, which serve as a significant reservoir of this bacteria and also a major hazard source for raw beef. Third-generation cephalosporin-resistant E. coli is one of the major disease causing bacteria in humans. The present study was designed to assess the third-generation cephalosporin-resistant E. coli contaminant of raw beef. Total 120 raw beef samples were collected from different butcher shops in retail market of Faisalabad, Pakistan. For sample preparation 25g raw beef samples were separated from every 120 samples and placed all these separated 25g samples on separate ziplock plastic bags having 225ml brain heart infusion broth in it and homogenised using a stomacher at 230rpm for 2 minutes. Isolation of E. coli was done by plating of 100 microliter sample on MacConkey agar, for pure culture, subcultured on Blood agar and for identification Grams staining, oxidase test, Indole test, Methyl Red test, Voges Proskauer test and catalase test were performed. Kirby-Baur disc diffusion method was performed for assessment of third generation cephalosporin resistant E. coli, antimicrobial discs such as ceftazidime, cefotaxime, ceftizoxime, ceftriaxone, cefixime, cefpodoxime, cefdinir and ceftibuten were placed on E. coli swabbed Mueller Hinton Agar. Out of 120 samples 80.83% samples were contaminated with E. coli from which E. coli showed resistant to Ceftriaxone 57.7%, Ceftazidime 61.8%, Cefotaxime 55.5%, Ceftibuten 41.2%, Cefixime 53.6%, Ceftizoxime 39.2%, Cefdinir 17.5% and Cefpodoxime 25.8%. Beef samples from local market of Faisalabad were significantly (p?0.05) more frequently contaminated with third generation cephalosporin resistant E. coli.

Keywords: E. coli, Raw beef, Cephalosporin resistance, Faisalabad