Abstract

Rapid recovery of Salmonella from chicken meat and poultry fecal samples by selective pre-enrichment

Authors: Mudassir Saleem, Habib-ur-Rahman and Juon Abbas

DOI: http://dx.doi.org/10.71081/cvj/2023.007

Abstract
Salmonella is Gram negative; rod shape and non-lactose fermenter bacteria belong to Enterobacteriaceae family. Two most important Serotypes of Salmonella enterica transmitted from animal to human. Salmonella cause pullorum disease in chicken and cause huge economic loss in poultry industries. Chicken and chicken meat products are major sources of Salmonella outbreaks. Salmonella detection based on culture-based protocol. Conventional method is time consuming take 4-5 days for isolation of Salmonella. In this study Salmonella recovered from chicken meat and fecal samples by culturing samples in pre-enrichment media that contain selective chemicals that enhance the growth of Salmonella only and retard the growth of another micro biota. Chicken meat samples were collected from different chicken meat shops and poultry fecal sample were collected from poultry farm. Samples were treated with selective pre enrichment broth. Biochemical test was performed for conformation for isolation of Salmonella. Selective pre-enrichment method is efficient for rapid recovery of Salmonella. Disc diffusion method was used to check antibiotic susceptibility of isolates. The salmonella isolates that were recover from poultry feces were more resistant to antibiotics than those isolates from chicken meat.

Keywords: Salmonella, Chicken meat, Poultry fecal samples, Bile salt, Novobiocin