Abstract

Antimicrobial resistance and virulence determinants of E. coli in bovine clinical mastitis in dairy farms

Authors: Sikander Mehmood, M. Ashraf and Muhammad Tahir Sarfraz Khan

DOI: http://dx.doi.org/10.71081/cvj/2023.008

Abstract
Escherichia coli (E. coli) is a significant pathogen that causes mastitis in bovine animals and decreases milk quality and production of milk. E. coli causes economic losses in the dairy industry all over the world, due to antimicrobial resistance and virulent genes (stx, eae and cdt) present in E. coli. The present study was conducted to examine the virulence factors, antimicrobial susceptibility, and virulent genes in E. coli. Samples were collected from buffaloes from different dairy farms in Faisalabad Punjab, Pakistan. Different techniques were applied for the identification and Isolation of E. coli. In the biochemical test Citrate test, Indole test, Gram staining technique, and motility test were used. For virulent genes (stx, eae and cdt) and detection PCR technique was used. For the detection of antimicrobial Resistance, the Kirby-Bauer disc diffusion method was used. After the experimentation process, results were statically analyzed by using CRD about the prevalence, virulence, and antimicrobial resistance of E. coli in the bovine animals in the Faisalabad Division. After experimentation and statically analyzed results, it was observed that E. coli was present in the collected samples in bovine animals. Antimicrobial resistance was high against E. coli. For the determination of virulence genes (stx, eae and cdt) PCR technique was used. It was observed after the PCR technique that stx gene was present in E. coli.

Keywords: E. coli, Mastitis, Bovine animals, Milk, PCR