Abstract

Isolation, characterization and preparation of inactivated vaccine against avian Infectious Bronchitis virus

Authors: Minahil Sajid, Atta ur Rehman and Misbah Ijaz

DOI: http://dx.doi.org/10.71081/cvj/2024.001

Abstract
The goal of this research is to isolate and identify the infectious bronchitis virus in order to create an inactivated vaccine. Infectious bronchitis is a very contagious upper respiratory disease that affects young poultry. Infectious bronchitis virus (IBV), the chicken coronavirus, is one of the leading causes of economic loss in the poultry business, affecting both meat-type and egg-laying birds. It is a member of the Coronavirus family, the genus Gamma coronavirus, which has a single-stranded RNA genome surrounded by a nucleocapsid and envelope. Clinical samples (trachea, bronchi, and caeca) were collected from the Clinical Diagnostic Lab of UAF. The samples were screened by HA, HI test for the presence of virus. IHA was performed for IBV because IBV has no glycoprotein on their surface. That's why add some mask reagent to form agglutination. The positive IBV samples were cultured in specific pathogen-free (SPF) embryonated hen's eggs. Virus isolation was performed by inoculation of 9-11-day-old eggs with 200l of 10% tissue homogenates and incubation at 37℃ for 72 hours. For vaccine, the confirmed virus was cultivated in embryonated chicken eggs. Sixty broiler chicks will be divided into 3 groups, having 20 each. First group was injected by the prepared vaccine. Second group was injected by a commercially available vaccine while the third was negative control. Standard statistical analysis was applied, and treatment means were estimated.

Keywords: IBV (Infectious Bronchitis Virus), SPF (specific pathogen-free), Vaccine, Poultry birds