Authors: Khadija Younas, Rana Aftab Ali Khan, Ali Numan, Hammad Ali, Ahsan Elahi, Aiman Khan, Rabia Zahid, Hassan Saeed, Kashif Iqbal, Muhammad Ali Huzaifa
DOI: http://dx.doi.org/10.71081/cvj/2024.014
Abstract
In order to maintain or enhance genetic diversity, particularly in endangered
species, essential procedures are applied in a variety of species, including
humans, livestock, and aquatic vertebrates and invertebrates. These
techniques include sperm cryopreservation and in vitro embryo creation.
Reactive oxygen species (ROS), which are molecules made from oxygen, are
frequently produced in greater amounts because of these techniques. ROS
levels in cells can range from low to high, leading to a variety of effects
including apoptosis, autophagy, and necrosis. To combat and neutralize
ROS, cells have intrinsic antioxidant systems made up of enzymes and non-
enzymatic antioxidants. Free radicals and oxidative stress have a big impact
on in vitro operations. To develop solutions to lessen their impacts, this
review focuses on analyzing how metallic and nonmetallic (NPs), as well as
their salts, regulate oxidative stress during in vitro embryo formation and
sperm cryopreservation.
Keywords: Reproductive, Population, Conservation