Abstract

Evaluation of Anti-Bacterial Efficacy of Nanoparticles Against Major Mastitis Associated Pathogens

Authors: Bilal Ahmed and Muhammad Asif Nawaz

DOI: http://dx.doi.org/10.71081/cvj/2024.030

Abstract
The present study reports the efficacy of zinc oxide nanoparticles (ZnO-NPs) and traditional antibiotics against E. coli and Staphylococcus aureus (S. aureus). The results showed that ZnO-NPs, regardless of concentration, did not demonstrate considerable antibacterial action against E. coli on their own but ZnO-NPs had a strong antibacterial action against S. aureus at different doses, suggesting that they may be employed to treat illnesses brought on by these bacteria. The study assessed the efficacy of traditional antibiotics, such as ciprofloxacin, and demonstrates that they had a strong antibacterial impact on S. aureus and E. coli. There were no appreciable variations in the antibacterial effectiveness of the nanoparticles at concentrations of 30 µL, 60 µL, and 120 µL, according to statistical analysis performed with Tukey's test through SPSS software. The concentration of ciprofloxacin alone and the combination of ciprofloxacin and ZnO-NPs, on the other hand, showed substantial differences, suggesting that the latter combination was not more effective. Furthermore, as compared to employing ZnO-NPs or antibiotics alone, the study showed that combining ZnO-NPs with conventional antibiotics has a decreased but still substantial antibacterial impact against both E. coli and S. aureus. In particular, the antibacterial action was much decreased when ciprofloxacin and ZnO-NPs were combined, especially against E. coli. The study's overall findings highlighted the possible advantages of ZnO-NPs antibacterial activity having more alone against S. aureus but in combination with traditional antibiotics were somehow reduced against bacteria. These results implied that enhancing the formulations of nanoparticles may be a viable tactic to fight bacterial infections and deal with the expanding problem of antibiotic resistance bacteria.

Keywords: Bovine mastitis, E. coli, Staphylococcus aureus, Zinc oxide nanoparticles, (ZnO-NPs).