Abstract

Comparative Effectiveness of Calcium Zincate Nanocomposite and Zinc Oxide Nanoparticles as Healing Agent Against Third Degree Burn Wound in Rabbits

Authors: Ahmad Raza, Muhammad Ijaz Saleem, Syed Faisal Hussain, Ashar Mahfooz, Muhammad Kashif Saleemi, Ambreen Ashar, Kashif Zafar, Abrar Ahmad, Fazeela Zaka, Asif Ali Butt, Syed Khalil ud Din Shah, Qari Muhammad Kaleem, Sahar Mustafa, Tahir Sultan, Atiq ur Rehman, Bilal Hassan and Muhammad Waseem Saleem

DOI: http://dx.doi.org/10.71081/cvj/2024.031

Abstract
The aim of this study was to evaluate the comparative healing effects of calcium zincate nanocomposite and zinc oxide nanoparticles on 3rd degree burn wound in rabbits. For this purpose, 4 groups were made and each group contained 4 rabbits (10 to 12 weeks old and weighing about 1.5 to 2.0kg). Group A kept as a control group was treated by normal saline (T0). Group B and C were kept as experimental groups (T1, T2) and were treated with 1% calcium zincate nanocomposite and 1% Zinc oxide nanoparticles, respectively. While, group D named as standard group (T3) was given Quench cream® 15g (Silver sulfadiazine 1% ointment). Factors considered for the testing in this experimental work were wound contraction, healing time, hematology, thickness of epidermis and histopathology of the burn wound. The results indicated that wound contraction was maximum and rapid in case of 1% Calcium zincate nanocomposite than 1% zinc oxide nanoparticles and Quench cream® 15g. 1% calcium zincate nanocomposite took less time to completely heal the wound than 1% Zinc oxide nanoparticles. 1% Zinc oxide nanoparticles showed good healing than the Quench® cream. Moreover, 1% calcium zincate nanocomposite had the highest collagen percentage than 1% zinc oxide nanoparticles. Tissues treated with 1% calcium zincate nanocomposite exhibited better tensile characteristics than tissues treated with zinc oxide nanoparticles and 1% silver sulfadiazine. Furthermore, 1% calcium zincate nanoparticles exhibited faster re-epithelization at the wound site in comparison to zinc oxide nanoparticles and 1% silver sulfadiazine. So, it is concluded that 1% calcium zincate nanocomposite give better wound healing effects than 1% zinc oxide nanoparticles.

Keywords: Calcium zincate, Nanocomposite, Healing time, Histopathology, Third-degree burn wound, Wound contraction rate, Zinc oxide nanoparticles,